12.5  HþME Tubing Cleaning Procedure

Peristaltic pump tubing was cleaned as described below.  All tubing was new Tygon except for approximately two inches of new Teflon tubing used to connect the filter to the Tygon tubing.  Cleaning solutions were pumped through the tubing with a peristaltic pump.

• Rinse outside and inside of tubing with Alconox and distilled water solution.
• Rinse inside and outside with distilled water.
• Rinse inside with nitric acid and HPLC[1] water solution (10% nitric acid).
• Rinse inside with distilled water.
• Rinse inside with HPLC water.

12.6  HþME Sample Collection Procedure

Between January 13^th and January 16^th, 2006, samples were collected from eight wells and two springs.  The samples were collected by George Rice, John Hadder, and Jennifer Viereck. The sampling procedure is described below.

• A minimum of three bore volumes were purged from each well before any sample water was collected. Bore volumes were calculated based on information provided by the well owner (total depth, depth to water, diameter).

·         Water was pumped from each well by the owner’s submersible pump.

·         Samples were collected from brass or plastic faucets a few feet from the wellhead. A few faucets were as far as 25 feet from the wellhead. In one case water was collected from a PVC pipe 125 feet from the wellhead.

·         Storage tanks were bypassed. Water flowed from the well, through a PVC pipe, to the faucet or end of the pipe. In some cases faucets were connected to a short length of galvanized steel pipe.

• At each well, a new, pre-cleaned one-liter HDPE[2] bottle was filled with sample water.
• Water was pumped from the HDPE bottle into the sample containers with a peristaltic pump.
• New, pre-cleaned, peristaltic pump tubing (Tygon) was used at each sample location.
• All samples were filtered through an in-line, 0.45 μm, disposable filter.
• All sample containers were new, pre-cleaned bottles.  Metals and most radionuclide samples were collected in HDPE bottles.  Each HDPE bottle contained a small amount of nitric acid.  The nitric acid was added by the analytical laboratory or the bottle supplier.  A combined tritium and carbon-14 sample was collected in a 500 ml, pre-cleaned glass bottle.  The glass bottle contained no nitric acid.  An additional 50 ml sample was collected in a plastic vial.  This sample was collected at the request of the analytical laboratory.  The vial contained no nitric acid.
• Spring samples were collected as described above except that the intake end of the tygon tubing was submerged directly in the spring pool.
• Temperature, pH, and electrical conductivity were measured at each sample location.

Additional information regarding the collection of samples at each location can be found on the sample collection forms.

12.7  Temperature Conversion Chart